Under these conditions, if no expiration date is mentioned on the kit label, qiaprep miniprep kits can be stored for up to 12 months without showing any reduction in performance and quality. We initially tried two different commercial miniprep kits. Qiaprep miniprep handbook 052012 5 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. The procedure combines a unique buffer system with zymospin column technology to yield hig. We reserve the right to change, alter, or modify any product to enhance its performance. Purifying plasmid dna from bacterial colonies using the qiagen miniprep kit. Plasmids and cosmids up to 50 kb in size can be purified using hispeed plasmid kit. Centrifuge for 15 seconds to elute the plasmid dna. This kit can also be used for dna cleanup from enzymatic reactions see page 8. Qiaprep miniprep kits should be stored dry at room temperature 1525c. Samples were analyzed on a bioanalyzer pico chip, with rin values and total yields shown below the lanes a. Plasmid dna encoding constitutively expressed gfp pegfpc2 was prepared using either monarch plasmid miniprep kit or qiagen qiaprep spin miniprep kit. Dna binding capacity of gencatchtm plus mini column is up to 40. Qiaprep miniprep kits is monitored routinely on a lottolot basis.
Genespin miniprep plasmid purification kit v2 for purification of highquality plasmid dna ver. T2006 monarch nuclease free water ml 25c quick protocol card introduction the monarch total rna miniprep kit is a comprehensive solution for sample preservation, cell lysis, gdna removal, and purification of total rna from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. The qiaprep spin miniprep kit can be fully automated on the qiacube. For minipreps, a single colony should be inoculated into 3 ml o. The quick dnarna miniprep plus kit combines quickdnarna technology with the addition of dnarna shield, a unique preservation and lysis technology, and proteinase k to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. For technical assistance, please call tollfree 0080022446000, or find regional phone. Qiaquick pcr purification kit 250 from qiagen selectscience. Mar 03, 2021 qiagen robotic systems are not available in all countries, please inquire. Compare qiaprep spin miniprep kit from qiagen sample to insight on catalog number. This protocol is designed for preparation of up to 100 g of high or lowcopy plasmid or cosmid dna using the qiagen plasmid midi kit, or up to 500 g using the. Grow yeast strain containing desired plasmid overnight in 10 ml of rich media yepd. This is the protocol for qiagens qiaprep spin miniprep kit catalog numbers 27104 or 27106 with their newer version 2.
The purchaser must determine the suitability of the product for its particular use. Two highcopy plasmids with different backbones were purified using purelink expi and qiagen endofree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Cap the microcentrifuge tube, and store eluted plasmid dna at 20c. For technical assistance, please call toll free 0080022446000, or find regional phone. Purelink expi endotoxinfree maxi plasmid purification kit. Purified plasmid dna is suitable for all routine downstream applications including bacterial cell transformation, mammalian cell transfection, dna sequencing, restriction enzyme digestion, cloning, and pcr. Pellet cells in 15 ml falcon tube, resuspend in water, and transfer to 1. All due care and attention should be exercised in the handling of the products. Qiaquick gel extraction kit protocol using a microcentrifuge.
Up to 400 mg agarose can be processed per spin column. The quick dnarna miniprep kit provides a quick method for the isolation of high quality genomic dna and total rna from small amounts of cells and tissue. University of guelph biobar 2020 price list jan 1 through dec. Qiagen plasmid purification handbook harvard university. The qiaprep spin miniprep kit is designed for isolation of up to 20. Purifying plasmid dna from bacterial colonies using the qiagen. They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. Resuspend pelleted bacterial cells in 250 l buffer p1 kept. Achieve high yields of endotoxin free, advanced transfectionquality plasmid dna with purelink expi when compared to kits from a competitor. Qiagen tips can be left unattended, since the flow of buffer will stop when the meniscus reaches the upper frit in the column. Qiagen qiaprep spin miniprep kit 27104 or 27106 with a. Qiagen plasmid kits should be stored at room temperature 1525c. The standard protocol on pages 911 of this handbook is for purification of up to 20 g. Alternatively, qiagen tip 20 may be placed in qiarack 1 figure 1b.
The qiaprep spin miniprep kit is designed for quick and convenient processing of 124 samples simultaneously in less than 30 minutes. Gencatchtm plus plasmid dna miniprep kit provides a simple, fast and costeffective method to purify plasmid dna without phenolchloroform extraction. We recommend all users of qiagen products to adhere to the nih guidelines that have. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Qiagen tips may be placed in tubes using tip holders provided with the kits figure 1a. The purelink expi endotoxin free maxi plasmid purification kit enables quick and simple isolation of endotoxin free plasmid dna using an enhanced anion exchange membrane. This protocol is designed for purification of total dna from grampositive bacteria. Zyppy miniprep kits result in the highest transfection efficiencies. Forefront tower ii 1, kachidoki 3 chome chuoku, tokyo 1040054. Quick start procedure this quick start procedure is for. I registered my instrument in my qiagen but it is not yet available in my dashboard. The dna yield of qiaprep miniprep kits is tested by preparing highcopy plasmid dna from 15 ml overnight culture in lb medium and assaying the dna yield.
Qiaprep miniprep handbook washington state university. Plasmid isolation miniprep adapted from qiagen miniprep handbook p1. These products arenotintendedforthediagnosis,prevention,ortreatmentofadisease. Qiagen plasmid kits are intended for molecular biology applications. Via grosio, 1010 20151 milano orders 0233430411 fax 0233430426 technical 0233430414 japan qiagen k. Endofree plasmid kits provide rapid, anionexchangebased endotoxin free plasmid dna purification. These are available online in convenient and compact pdf format at. Print bookmark share pdf 2487kb english format file size language download get adobe reader contact qiagen.
Qiagen will replace it free of charge or refund the purchase price. Qiaprep products are dependable and time efficient. Qiaprep spin miniprep kit 250 from qiagen selectscience. It is based on binding of dna to silicabased membranes in chaotropic salts.
The procedure combines a unique buffer system with zymospin column technology to yield high quality total rna including small rnas 17200 nt in about 10 minutes. Should any product fail to perform satisfactorily due to any reason other than misuse, qiagen will replace it free of charge or refund the purchase price. The quickrna miniprep kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from a wide range of cell up to 107 and tissue samples up to 50 mg. Plasmid dna purified with qiagentips is free of all contaminants, and ideally. Endotoxinfree plasmid dna isolation kits thermo fisher. Na0600 genelute hp endotoxin free plasmid megaprep kit 5 preps na0800 genelute hp select plasmid gigaprep kit 5 preps to reorder product call 18003253010.
The qiaprep miniprep system provides a fast, simple, and costeffective plasmid miniprep method for routine molecular biology laboratory applications. For a limited time, free trials of our new rna cleanup kits are also available. Kits can be stored for up to 12 months without showing any reduction in performance and quality. Purifying plasmid dna from bacterial colonies using the. Qiaprep spin miniprep kit protocol for yeast using a microcentrifuge procedure 1. This protocol is designed for purification of up to 20. Setup of qiagen tips awith tip holder or bwith qiarack 1. The protocol allowed for higher yield by reducing the amount of elution. Qiafilter cartridges enable fast lysate clearing by filtration. Qiagen miniprep protocol pellet 15ml of an overnight culture by spinning 1. The protocol uses 2ml of an overnight bacterial culture. These are available online in convenient and compact pdf.
For spincolumn or 96well extraction of total dna from animal blood and tissues and from cells, yeast, bacteria, or viruses. The standard protocol on pages 1819 should be followed with the following modifications. In accordance with qiagen s isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. For larger constructs qiagen plasmid kits or the largeconstruct kit are recommended plasmidcosmid copy number plasmid and cosmids vary in copy number, depending on the origin of replication they contain, their size, and the size of insert.
Qiagen tips should be stored dry and at room temperature 1525c. The purified dna exceeds the purity obtained by 2 x cscl gradient centrifugation and is suitable for advanced transfectiongrade applications. Plasmid dna purification using the qiaprep spin miniprep kit and a. Qiaprep spin miniprep kit protocol using 5ml collection tubes the qiaprep spin miniprep procedure can be performed using 5ml centrifuge tubes e. The quickrna miniprep kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from a wide range of cell up to 10 7 and tissue samples up to 50 mg. After adding rnase a, buffer p1 should be stored at 28c and is stable for 6. Four different cell lines cos7, hek293, hela, and huh7 were grown to 8090% confluence and transfected with 100 ng of each plasmid, in complex with 0.
This new plasmid dna isolation protocol will significantly reduce time and labor. Qiaprep miniprep kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. The dna yield of qiaprep miniprep kits is tested by preparing highcopy plasmid dna from. This protocol is designed for purification of up to 20 g of highcopy plasmid dna from. I have found that 2ml epptubes work best for this part of the procedure. The dna yield of qiaprep miniprep kits is tested by preparing highcopy plasmid dna from 15 ml overnight. They are not to be used for human diagnostic or drug purposes or to be administered. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Qiaprep miniprep kits are developed, designed, and sold for research purposes only. University of guelph biobar 2020 price list jan 1 through. Please do not hesitate to contact us if you have any questions. Dna binding capacity is tested by determining the recovery obtained with 10 g and 20 g of various plasmid dnas.
Qiagen guarantees the performance of all products in the manner described in our product literature. This protocol is for purification of up to 100 g endotoxin free plasmid dna using qiagen tip 100. The kit isolates both genomic dna and a broad range of rna species without the use of phenol. Luciferase ities were determined in lysates from cells transfected with dna isolated by various methods. Isolation of endotoxinfree plasmid dna using the qiagen.
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